# behav: apropos of the uncertainty about the 'Zona Incerta', a thresholding mechanism involved in curiosity and novelty-seeking behavior

<< Motivational drives are internal states that can be different even in similar interactions with external stimuli. Curiosity as the motivational drive for novelty-seeking and investigating the surrounding environment is for survival as essential and intrinsic as hunger. Curiosity, hunger, and appetitive aggression drive three different goal-directed behaviors—novelty seeking, food eating, and hunting >>️

<< Optogenetic activation of inhibitory neurons in medial ZI (ZIm) [medial Zona Incerta in mouse, freedom to choose what it wants], ZImGAD2 neurons, showed a dramatic increase in positive arousal level, depth of investigation, and duration of interaction with conspecifics and novel objects compared with familiar objects, crickets, and food. Optogenetic or chemogenetic deactivation of these neurons decreased depth and duration of investigation. Moreover, [AA] found that ZImGAD2 neurons are more active during deep investigation as compared with during shallow investigation. >>

<< [They] found that activation of prelimbic cortex (PL) axons into ZIm increases arousal level, and chemogenetic deactivation of these axons decreases the duration and depth of investigation. >>

<< Optogenetic activation of ZImGAD2 axons into lateral periaqueductal gray (lPAG) increases the arousal level, whereas chemogenetic deactivation of these axons decreases duration and depth of investigation. Calcium fiber photometry of these axons showed high activity during deep investigation and no significant activity during shallow investigation, suggesting a thresholding mechanism. >>️

<< Last, [AA] found a new subpopulation of inhibitory neurons in ZIm expressing tachykinin 1 (TAC1) that monosynaptically receive PL inputs and project to lPAG. Optogenetic activation and deactivation of these neurons, respectively, increased and decreased depth and duration of investigation. >>️️

Ahmadlou, Janou H. W. Houba, et al. A cell type–specific cortico-subcortical brain circuit for investigatory and novelty-seeking behavior. Science . Vol. 372, Issue 6543, eabe9681. doi: 10.1126/ science.abe9681. May 14, 2021. 

https://science.sciencemag.org/content/372/6543/eabe9681   

Brain mechanism of curiosity unraveled. Netherlands Institute for Neuroscience - KNAW.  May 13, 2021. 

https://www.sciencedaily.com/releases/2021/05/210513142430.htm