# gene: point code cracking machines (without detectable byproducts)

DNA cracking machine

<< The spontaneous deamination of cytosine is a major source of C•G to T•A transitions, which account for half of known human pathogenic point mutations. The ability to efficiently convert target A•T base pairs to G•C could therefore advance the study and treatment of genetic diseases >>

AA << report adenine base editors (ABEs) that mediate conversion of A•T to G•C in genomic DNA >>

<< ABEs advance genome editing by enabling the direct, programmable introduction of all four transition mutations without double-stranded DNA cleavage >>

Nicole M. Gaudelli, Alexis C. Komor, et al. Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 2017 doi: 10.1038/nature24644 Oct 25, 2017
   
https://www.nature.com/nature/journal/vaap/ncurrent/full/nature24644.html

<< there are virtually no detectable byproducts such as random insertions, deletions, translocations, or other base-to-base conversions >>

Researchers extend power of gene editing by developing a new class of DNA base editors. Oct 25, 2017

https://m.phys.org/news/2017-10-enzyme-rewrites-genome.html

RNA cracking machine

<< RNA Editing for Programmable A to I Replacement (REPAIR), which has no strict sequence constraints, can be used to edit full-length transcripts containing pathogenic mutations >>

David B. T. Cox, Jonathan S. Gootenberg, et al. RNA editing with CRISPR-Cas13. Science. Oct 25, 2017:eaaq0180 doi: 10.1126/science.aaq0180

http://science.sciencemag.org/content/early/2017/10/24/science.aaq0180

Researchers engineer CRISPR to edit single RNA letters in human cells. Oct 25, 2017

https://m.phys.org/news/2017-10-crispr-rna-letters-human-cells.html

Lauran Neergaard. Scientists working toward reversible kind of gene editing. Oct. 25, 2017

https://m.phys.org/news/2017-10-scientists-reversible-kind-gene.html

also:

http://flashontrack.blogspot.it/search?q=crispr